Optimized Expression of Full-Length IgG1 Antibody in a Common E. coli Strain
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چکیده
منابع مشابه
Optimized Expression of Full-Length IgG1 Antibody in a Common E. coli Strain
Multi-polypeptide proteins such as antibodies are difficult to express in prokaryotic systems such as E. coli due to the complexity of protein folding plus secretion. Thus far, proprietary strains or fermenter cultures have been required for appreciable yields. Previous studies have shown that expression of heterologous proteins in E. coli can be enhanced by the reduction of protein translation...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2010
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0010261